Pathology Specimen Handbook

Recent changes for all departments


 Recent changes for ACYL CARNITINES

Recent changes for ACYL CARNITINES
DateFieldChanged FromChanged To
5th August 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
Optional Container TypeHeparin Plasma (Gel)

 Recent changes for ADENOSINE DEAMINASE ACTIVITY

Recent changes for ADENOSINE DEAMINASE ACTIVITY
DateFieldChanged FromChanged To
27th August 2019Request GroupADEN (For Blood) ZADEN (For Fluids)ADEN (For Blood)
Optional Specimen TypeFluid
Optional Container TypeHeparin Whole Blood (No Gel)
Collection & Request Instructions

Pleural fluid may be used for this assay.

Pleural fluid is stable for 7 days at 4 C, spin and send supernatant at 4 C.

 

 

 

 Recent changes for AMYLASE (24 HOUR URINE)

Recent changes for AMYLASE (24 HOUR URINE)
DateFieldChanged FromChanged To
31st July 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
26th July 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL

 Recent changes for AMYLASE (TIMED URINE)

Recent changes for AMYLASE (TIMED URINE)
DateFieldChanged FromChanged To
31st July 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL

 Recent changes for BILIRUBIN (NEONATE)

Recent changes for BILIRUBIN (NEONATE)
DateFieldChanged FromChanged To
9th July 2019Collection & Request InstructionsProtect from light

 Recent changes for BRAF V600E mutation test (melanoma)

Recent changes for BRAF V600E mutation test (melanoma)
DateFieldChanged FromChanged To
14th November 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
Collection & Request Instructions

Internal requests - BRAF molecular testing is requested in reflex manner on a 'Molecular Oncology Request Form' by the reporting Anatomical Pathologists. Non-pathology staff (i.e. oncologists, surgeons, etc) should contact the reporting Anatomical Pathologist if BRAF molecular test needs to be added to a case. 

External requests - GMP laboratory will only process requests for BRAF testing for patients who had their histology performed at Monash Pathology. Please provide patient's Medicare number for billing purposes. MBS applies to Stage III and IV melanoma. Tests on other tumour types will be charged at the MBS schedule rate.

If your request is not covered by the above categories please contact the laboratory on 03 9594 3840.

 

 

Internal requests - BRAF molecular testing is requested in a reflex manner on a 'Molecular Oncology Request Form' by the reporting Anatomical Pathologists. Non-pathology staff (i.e. oncologists, surgeons, etc) should contact the reporting Anatomical Pathologist if BRAF molecular test needs to be added to a case. 

External requests - GMP laboratory will only process requests for BRAF testing for patients who had their histology performed at Monash Pathology. Please provide patient's Medicare number for billing purposes. MBS applies to Stage III and IV melanoma. Tests on other tumour types will be charged at the MBS schedule rate.

If your request is not covered by the above categories please contact the laboratory on 03 9594 3840.

 

 

14th November 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
Collection & Request Instructions

Requesting clinicians must clearly indicate the test requested with appropriate clinical information. Clinical information must include the accession number of the histology episode on which the test is to be performed and any other relevant clinical information. Please fax requests to Monash Pathology on 9594 6619. Specimens will be retrieved from Histology and forwarded to Genetics and Molecular Pathology for testing. Monash Pathology will only process requests for patients who have had their histology performed at Monash Pathology. To avoid the patient receiving a bill for this test the patient’s  Medicare number with the patient’s signature must be provided. The MBS currently only applies for tests on Stage III and IV melanoma. Tests on other tumours will be charged at the MBS schedule rate.

 

Internal requests - BRAF molecular testing is requested in reflex manner on a 'Molecular Oncology Request Form' by the reporting Anatomical Pathologists. Non-pathology staff (i.e. oncologists, surgeons, etc) should contact the reporting Anatomical Pathologist if BRAF molecular test needs to be added to a case. 

External requests - GMP laboratory will only process requests for BRAF testing for patients who had their histology performed at Monash Pathology. Please provide patient's Medicare number for billing purposes. MBS applies to Stage III and IV melanoma. Tests on other tumour types will be charged at the MBS schedule rate.

If your request is not covered by the above categories please contact the laboratory on 03 9594 3840.

 

 

 Recent changes for CATECHOLAMINES (24 HOUR URINE)

Recent changes for CATECHOLAMINES (24 HOUR URINE)
DateFieldChanged FromChanged To
1st July 2019Request GroupUMETAN UCRT (Include UCATM if a paediatric sample)UMETAN UCRT
Volume (Adults)null mL
Minimum/Paediatric Volumenull mL

 Recent changes for CATECHOLAMINES (SPOT URINE)

Recent changes for CATECHOLAMINES (SPOT URINE)
DateFieldChanged FromChanged To
11th July 2019Request GroupUMETAN UCRS (Include UCATMCR if a paediatric sample)UMETAN UCRS

 Recent changes for CATECHOLAMINES (TIMED URINE)

Recent changes for CATECHOLAMINES (TIMED URINE)
DateFieldChanged FromChanged To
11th July 2019Request GroupUMETAN UCRT (Include UCATM if a paediatric sample)UMETAN UCRT
Volume (Adults)null mL
Minimum/Paediatric Volumenull mL

 Recent changes for CEFAZOLIN

Recent changes for CEFAZOLIN
DateFieldChanged FromChanged To
19th July 2019Optional Container TypeHeparin Plasma (No Gel)
Serum (No Gel)
Heparin Plasma (No Gel)

 Recent changes for CEPHALEXIN

Recent changes for CEPHALEXIN
DateFieldChanged FromChanged To
19th July 2019Optional Container TypeHeparin Plasma (No Gel)

 Recent changes for Creutzfeld Jacob Disease

Recent changes for Creutzfeld Jacob Disease
DateFieldChanged FromChanged To
12th August 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL

 Recent changes for DNA - CONNEXIN 26

Recent changes for DNA - CONNEXIN 26
DateFieldChanged FromChanged To
13th November 2019Request GroupMISCGENGMPTRAN
Minimum/Paediatric Volumenull mL

 Recent changes for DNA - MYOTONIC DYSTROPHY

Recent changes for DNA - MYOTONIC DYSTROPHY
DateFieldChanged FromChanged To
29th August 2019Alternative namesMYOTONIC DYSTROPHYMYOTONIC DYSTROPHY, DM1 Gene
Request GroupMISCGENGMPTRAN

 Recent changes for FATTY ACIDS - ESSENTIAL

Recent changes for FATTY ACIDS - ESSENTIAL
DateFieldChanged FromChanged To
22nd November 2019Minimum/Paediatric Volumenull mL
Preferred Container TypeEDTA PlasmaSerum - Gel tube - Contains Clot Activator
Optional Container TypeSerum (No Gel)EDTA Plasma
Heparin Plasma (Gel)
Collection & Request Instructions

Patient should fast 12 hours prior to sample collection.

Patient should fast 12 hours prior to sample collection.

The patient must not consume any alcohol for 24 hours before the specimen is drawn.

 Recent changes for FATTY ACIDS - FREE

Recent changes for FATTY ACIDS - FREE
DateFieldChanged FromChanged To
13th September 2019Preferred Container TypeFluoride OxalateFluoride Oxalate
Serum - Gel tube - Contains Clot Activator

 Recent changes for FOLATE (RED CELL)

Recent changes for FOLATE (RED CELL)
DateFieldChanged FromChanged To
25th September 2019Minimum/Paediatric Volumenull mL
Collection & Request Instructions

SERUM folate is the prefered test for folate status.

Red Cell Folate will only be done if specifically justified after discussion with the Clinical Haematologist or Chemical Pathologist.

SERUM folate is the prefered test for folate status.

 

 Recent changes for GASTRIN

Recent changes for GASTRIN
DateFieldChanged FromChanged To
15th July 2019Request GroupGASTGAST, MISCBIO
FrequencyWeekly

 Recent changes for HERPES SIMPLEX VIRUS (HSV- type 1/2) PCR

Recent changes for HERPES SIMPLEX VIRUS (HSV- type 1/2) PCR
DateFieldChanged FromChanged To
20th October 2019Assay nameHERPES SIMPLEX VIRUS (HSV- type 1/2) PCRPNEUMOCYSTIS JIROVECI (PJP) PNEUMONIA
Alternative namesHSV PCR, HERPES PCR, HERPES SIMPLEX VIRUS (HSV- type 1 2) CULTURE / IMMUNOFLUORESCENCE, HSV CULTURE/IFPNEUMOCYSTIS CARINII, PCP, PNEUMOCYSTOSIS
Request GroupHEAPCRPJPPCR
Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
Preferred Specimen TypeSwab
Blood
Cerebro Spinal Fluid (CSF)
Induced Sputum
Bronchoalveolar Lavage
Bronchial Washings
Optional Specimen Typerefer to collection instructions
Preferred Container TypeFlocked swab with UTM
PPT
EDTA Whole Blood
Sterile Container
Collection & Request Instructions

Herpetic lesion: Wipe vesicle with saline. Disrupt vesicle, and collect fluid with dry PCR swab. With the same swab, collect cells from base of lesion. For non-vesicular lesions, collect cells from base of lesion by using swab moistened with saline. Specimens can be collected from vesicles from a variety of sites including genital. Refer to Herpes Multiplex PCR for CSF samples.

For other specimens collect as clinically indicated. This may include:  tissue, CSF, saliva swab, urine, BAL and blood (PPT tube for adults, EDTA for paediatrics). Blood samples require a dedicated blood tube.

Collect Bronchial Washings, BAL, Lung Biopsy or Induced Sputum into sterile container. Specific requests and clinical notes are required. Expectorated sputum is not an appropriate specimen for detection of Pneumocystis.

FrequencyMonday
Wednesday
Friday

 Recent changes for KEPPRA

Recent changes for KEPPRA
DateFieldChanged FromChanged To
22nd August 2019Preferred Container TypeSerum (No Gel)Heparin Plasma (No Gel)
Optional Container TypeHeparin Plasma (No Gel)
Collection & Request Instructions

Trough level 8 hours post dose

MUST be non gel serum tube

Trough (pre-dose) level 8 hours post oral dose

MUST be non gel lithium heparin tube

 Recent changes for LEUKAEMIA DNA ANALYSIS

Recent changes for LEUKAEMIA DNA ANALYSIS
DateFieldChanged FromChanged To
16th October 2019Alternative namesFIP1L1-PDGFRA, PDGFRA, TCR GENE REARRANGEMENT, IGH GENE REARRANGEMENT, FLT3, AML1-ETO (t8:21), BCL2 (t14:18), BCL1 (t11:14), CBFB-MYHII (Inv 16), INV 16 QPCR, C-KIT, NPM1, T CELL GENE REARRANGEMENT, TP53 Mutation, AML SEQUENOMFIP1L1-PDGFRA, PDGFRA, TCR GENE REARRANGEMENT, IGH GENE REARRANGEMENT, FLT3, AML1-ETO (t8:21), BCL2 (t14:18), BCL1 (t11:14), CBFB-MYHII (Inv 16), INV 16 QPCR, C-KIT, NPM1, T CELL GENE REARRANGEMENT, TP53 Mutation, AML SEQUENOM, t(15;17) PML-PARA

 Recent changes for LINEZOLID

Recent changes for LINEZOLID
DateFieldChanged FromChanged To
30th August 2019Collection & Request Instructions

Collect 2 EDTA tubes.

Collect 2 EDTA tubes just before the next dose (trough collection) at least 48 hours after initiation or dose change (to reach steady state).

 Recent changes for Miscellaneous molecular

Recent changes for Miscellaneous molecular
DateFieldChanged FromChanged To
9th July 2019Optional Specimen TypeTissue - Fixed (Formalin)
Tissue - Fresh
Tissue - Frozen

 Recent changes for MYCOBACTERIUM TUBERCULOSIS PCR for Non-Respiratory Samples

Recent changes for MYCOBACTERIUM TUBERCULOSIS PCR for Non-Respiratory Samples
DateFieldChanged FromChanged To
5th September 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
5th September 2019Alternative namesTB PCR, MTB PCR
Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
5th September 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
Preferred Specimen TypeTissue - Fresh
Fluid
Bone Marrow
Cerebro Spinal Fluid (CSF)
Tissue - Fresh
Fluid
Bone Marrow
Cerebro Spinal Fluid (CSF)
Urine

 Recent changes for MYCOBACTERIUM TUBERCULOSIS PCR for Respiratory Samples

Recent changes for MYCOBACTERIUM TUBERCULOSIS PCR for Respiratory Samples
DateFieldChanged FromChanged To
5th September 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL

 Recent changes for MYCOPHENOLATE

Recent changes for MYCOPHENOLATE
DateFieldChanged FromChanged To
12th July 2019Collection & Request Instructions

Sample must be collected on ice.

Pre dose measurements are preferred.

However some requests may require 4 samples for area under the curve calculation.

Pre dose  

1hr post dose

2hrs post dose

4 hrs post dose

Requesting doctors must fill in a questionnaire of patient information

https://monashpathology.org/wp-content/uploads/2019/07/Mycophenolic_Acid.pdf

Sample must be collected on ice.

Requesting doctors must fill in a questionnaire of patient information

https://monashpathology.org/wp-content/uploads/2019/07/Mycophenolic_Acid.pdf

11th July 2019Collection & Request Instructions

Sample must be collected on ice.

Pre dose measurements are preferred.

However some requests may require 4 samples for area under the curve calculation.

Pre dose  

1hr post dose

2hrs post dose

4 hrs post dose

Sample must be collected on ice.

Pre dose measurements are preferred.

However some requests may require 4 samples for area under the curve calculation.

Pre dose  

1hr post dose

2hrs post dose

4 hrs post dose

Requesting doctors must fill in a questionnaire of patient information

 Recent changes for PNEUMOCYSTIS JIROVECI PCR

Recent changes for PNEUMOCYSTIS JIROVECI PCR
DateFieldChanged FromChanged To
20th October 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
Collection & Request InstructionsCollect Bronchial Washings, BAL, Lung Biopsy or Induced Sputum into sterile container. Specific requests and clinical notes are required.

Collect Bronchial Washings, BAL, Lung Biopsy or Induced Sputum into sterile container. Specific requests and clinical notes are required.

 Recent changes for QUANTIFERON-TB GOLD PLUS ASSAY

Recent changes for QUANTIFERON-TB GOLD PLUS ASSAY
DateFieldChanged FromChanged To
4th July 2019Collection & Request Instructions

Requires QuantiFERON-TB Gold Plus Blood Collection Tubes set of four. Collect 1 mL into each tube. Refer to photograph for order of draw. Mix gently by turning tube end-over end X10 ensuring that the entire inner surface of the tube has been coated in blood.

Collections are to be performed at Pathology Collection rooms. Send to laboratory immediately after collection.

Please collect from Monday to Friday between 0900-1600 hours only.

DO NOT STORE IN FRIDGE BEFORE SENDING

Requires QuantiFERON-TB Gold Plus Blood Collection Tubes set of four. Collect 1 mL into each tube. Refer to photograph for order of draw. Mix gently by turning tube end-over end X10 ensuring that the entire inner surface of the tube has been coated in blood.

Collections are to be performed at Pathology Collection rooms. Send to laboratory immediately after collection.

Please collect from Monday to Friday between 0900-1600 hours only.

Please answer question if patient is immunosuppressed.

If non-MBS then non CMBS test

DO NOT STORE IN FRIDGE BEFORE SENDING

 Recent changes for RABIES (LYSSA VIRUS) SEROLOGY

Recent changes for RABIES (LYSSA VIRUS) SEROLOGY
DateFieldChanged FromChanged To
4th July 2019Collection & Request Instructions

Non-CMBS test

 Recent changes for RABIES PCR

Recent changes for RABIES PCR
DateFieldChanged FromChanged To
4th July 2019Collection & Request InstructionsRequires dedicated blood sample

Requires dedicated blood sample

Non-CMBS test

 Recent changes for STICKY TAPE TEST

Recent changes for STICKY TAPE TEST
DateFieldChanged FromChanged To
16th July 2019Alternative namesAnal Impression SmearsAnal Impression Smears; Pin Worm
Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
Collection & Request Instructions

Collect specimen from the perianal area first thing in the morning, before the patient has bathed or used the toilet. The test should be performed for at least  4-6 consecutive days with negative results before a patient is considered free of infection.

A. Place a segment of sticky tape (adhesive side down) on a microscope slide: starting 1.5cm from one end, run the tape towards the same end, and wrap the tape around the slide to the opposite end. Tear the tape even with the end of the slide. Attach a label to the tape at the end torn flush with the slide.

B. Peel back the tape by gripping the labeled end and with the tape looped (adhesive side outward) over a wooden tongue depressor that is held firmly against the slide and extended about 2.5cm beyond it, press the tape firmly several times against the right and left perianal folds.

C. Smooth the tape back on the slide, adhesive side down. 

Collect specimen from the perianal area first thing in the morning, before the patient has bathed or used the toilet. The test should be performed for at least  4-6 consecutive days with negative results before a patient is considered free of infection.

A. Place a segment of sticky tape (adhesive side down) on a microscope slide: starting 1.5cm from one end, run the tape towards the same end, and wrap the tape around the slide to the opposite end. Tear the tape even with the end of the slide. Attach a label to the tape at the end torn flush with the slide.

B. Peel back the tape by gripping the labeled end and with the tape looped (adhesive side outward) over a wooden tongue depressor that is held firmly against the slide and extended about 2.5cm beyond it, press the tape firmly several times against the right and left perianal folds.

C. Smooth the tape back on the slide, adhesive side down. 

 Recent changes for TESTING

Recent changes for TESTING
DateFieldChanged FromChanged To
9th December 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL

 Recent changes for TOXOPLASMA SEROLOGY

Recent changes for TOXOPLASMA SEROLOGY
DateFieldChanged FromChanged To
27th June 2019Request GroupTOXG,TOXMTOXGM (Acute), TOXG (IgG Screen only) TOXM (Infant ToRCH)
FrequencyDailyMon-Fri

 Recent changes for TREPONEMA PALLIDUM SEROLOGY

Recent changes for TREPONEMA PALLIDUM SEROLOGY
DateFieldChanged FromChanged To
27th June 2019FrequencyTwice weeklyMon-Fri
Uncontrolled when downloaded or printed.
Date printed: 14th December, 2019 12:30:07