Pathology Specimen Handbook

When Aldosterone and Renin requested together collect SST Yellow top serum tube for Aldosterone and EDTA Purple top tube for Renin. Refer to RENIN in the Pathology Specimen Handbook for collection instructions. Keep Renin tube at room temperature. Do not refrigerate.

Aldosterone and Renin can be collected in the same EDTA Purple tube.

For Aldosterone Renin ratio collect a SST Yellow top serum tube for Aldosterone and 2 EDTA Purple top tube for Renin. Refer to RENIN and Aldosterone in the Pathology Specimen Handbook for collection instructions. Keep Renin tube at room temperature. Do not refrigerate.

Refer to RENIN and Aldosterone for collection instructions

Send immediately on ice.

Please fill in the information form provided.

http://seals.rb.com.au/seals_tlm/store/Liverpool_Documents_ifx_final.pdf

Note: The Infliximab brand: Inflectra, Remicade or Other (Specify) must be noted on the form. The sample MUST NOT be sent without these details.

Send to laboratory IMMEDIATELY after collection� as the sample must be centrifuged and frozen within 1 hour of collection.

Please fill in the information form provided.

http://seals.rb.com.au/seals_tlm/store/Liverpool_Documents_ifx_final.pdf

Note: The Infliximab brand: Inflectra, Remicade or Other (Specify) must be noted on the form. The sample MUST NOT be sent without these details.

Sample is stable in blood for up to 7 hours at room temperature, and 24hours at 4°C.

Transport from collection centres in red bag on next courier run.

Transport from collection centres in red bag on next courier run.

MUST BE COLLECTED IN OWN TUBE

Sample is stable in blood for up to 7 hours at room temperature

Transport from collection centres in red bag on next courier run.

Sample is stable in blood for up to 7 hours at room temperature, and 24hours at 4°C.

Transport from collection centres in red bag on next courier run.

This test has been discontinued.

For enquiries, please contact the Chemical Pathologists.

This test has been discontinued.

For enquiries, please contact the Chemical Pathologist (Tel: 9594 4525)

All serology tests require a dedicated serum tube

All serology tests require a dedicated serum tube

Retesting is not required within 7 days or if IgG has previously been detected.

All serology tests require a dedicated serum tube

All serology tests require a dedicated serum tube

Retesting is not required within 7 days or if IgG has previously been detected.

If Hb is ordered following a Red Cell transfusion, it should be performed >15 minutes after the completion of the transfusion.

PLEASE NOTE: Clinicians must complete the ARCBS Indication for testing / clinical information form, which must be sent with samples

Link to ARCBS form  https://monashpathology.org/wp-content/uploads/2019/06/ARCBS_forms.pdf

DO NOT CENTRIFUGE EITHER EDTA OR SERUM SAMPLES- SEND AS WHOLE BLOOD TO VTIS.

(a)   If for PAB, collect 18 mL EDTA and 6ml of serum.

(b)   If for NAIT, FMAIT.  Collect 18mL of EDTA and 12mL Serum from the mother. Collect 18mL of EDTA from the father. Collect 1-2mL EDTA or buccal swab and 1-2mL serum from the child. Only available Mondays to Thursdays Must be BOOKED IN ADVANCE by DOCTOR Contact Red Cross Blood Bank ph: 96940354 prior to collection. Please notify a supervisor in Pathology - DO NOT REFRIGERATE OR FREEZE 

(c) If blood cannot be collected for platelet antibodies, an ORAcollect-DNA buccal swab may be used (stable 5 days in 4degC) 

PLEASE NOTE: Clinicians must complete the ARCBS Indication for testing / clinical information form, which must be sent with samples

Link to ARCBS form  https://monashpathology.org/wp-content/uploads/2019/06/ARCBS_forms.pdf

DO NOT CENTRIFUGE EITHER EDTA OR SERUM SAMPLES- SEND AS WHOLE BLOOD TO VTIS.

(a)   If for PAB, collect 6 mL ACD and 6ml of serum.

(b)   If for NAIT, FMAIT.  Collect 18mL of EDTA and 12mL Serum from the mother. Collect 18mL of EDTA from the father. Collect 1-2mL EDTA or buccal swab and 1-2mL serum from the child. Only available Mondays to Thursdays Must be BOOKED IN ADVANCE by DOCTOR Contact Red Cross Blood Bank ph: 96940354 prior to collection. Please notify a supervisor in Pathology - DO NOT REFRIGERATE OR FREEZE 

(c) If blood cannot be collected for platelet antibodies, an ORAcollect-DNA buccal swab may be used (stable 5 days in 4degC) 

Other specimen type: 0.5 mL CSF or Brain tissue. Blood samples requires a dedicated blood tube.

Transport to department at room temperature. * NOT AT 4oC.

Transport to department at room temperature only or frozen.

* NOT AT 4oC.

Serology for COVID-19 should only be ordered with appropriate clinical presentation. Please refer to Public Health Laboratory Network Guidance for serological testing in COVID-19 or contact the Microbiology registrar to discuss.

https://www.health.gov.au/sites/default/files/documents/2020/08/phln-guidance-for-serological-testing-in-covid-19-phln-guidance-on-serological-testing-in-covid-19.pdf

Baseline sample: 1x SST Gel tube (6 mL) for Basal Cortisol; 1x EDTA (4 mL) (sent on ice)for ACTH .

If 17-OH Progesterone is requested a Non-gel SST tube must be collected.

60 minute sample: 1 x SST Gel tube.

Short Synacthen Test should be booked with the Chemical Pathologist or Chemical Pathology Registrar.

1.Baseline sample: 1x SST 5ml Gel tube for Cortisol; 2 x EDTA 3 mL (1 will be accepted) sent on ice for ACTH

2.60 minute sample: 1 x SST Gel tube

3.If 17-OH Progesterone is requested a Non-gel SST tube must be collected with each tube point

4. Record height and weight

5. Short Synacthen Test should be booked with the Chemical Pathologist or Chemical Pathology Registrar.

Baseline sample: 1x SST Gel tube (6 mL) for Basal Cortisol; 1x EDTA (4 mL) (sent on ice)for ACTH .

If 17-OH Progesterone is requested a Non-gel SST tube must be collected.

60 minute sample: 1 x SST Gel tube.

Short Synacthen Test should be booked with the Chemical Pathologist or Chemical Pathology Registrar.

1. Baseline sample: 1 x 5ml SST Gel tube for Cortisol; 2 x 3ml EDTA sent on ice (1 tube will be accepted) for ACTH

2. 60 minute sample: 1 x SST Gel tube

3. If 17-OH Progesterone is requested a Non-gel SST tube must be collected

4. Record weight and height

5. Short Synacthen Test should be booked with the Chemical Pathologist or Chemical Pathology Registrar.

Baseline sample: 1x SST Gel tube (6 mL) for Basal Cortisol; 1x EDTA (4 mL) (sent on ice)for ACTH .

If 17-OH Progesterone is requested a Non-gel SST tube must be collected.

60 minute sample: 1 x SST Gel tube.

Short Synacthen Test should be booked with the Chemical Pathologist or Chemical Pathology Registrar.

1.Baseline sample: 1x SST Gel tube (5 mL) for Basal Cortisol; 2 x EDTA (3 mL) sent on ice for ACTH. One EDTA will be acceptable when 2 not available

2. 60 minute sample: 1 x SST gel tube (3ml)

3. If 17-OH progesterone is requested, a non gel SST tube must be collected with each tube point.

4. Record weight and height

5. Short Synacthen Test should be booked with the Chemical Pathologist or Chemical Pathology Registrar.

Baseline sample: 1x SST Gel tube (6 mL) for Basal Cortisol; 1x EDTA (4 mL) (sent on ice)for ACTH .

If 17-OH Progesterone is requested a Non-gel SST tube must be collected.

60 minute sample: 1 x SST Gel tube.

Short Synacthen Test should be booked with the Chemical Pathologist or Chemical Pathology Registrar.

1. Baseline sample: 1x SST Gel tube (5 mL) for Cortisol

2. 60 minute sample: 1 x SST Gel tube.

3. ACTH: 2 x EDTA 3ml (one tube will be accepted in difficult collections)

4. If 17-OH Progesterone is requested a Non-gel SST tube must be collected with each tube point

5. Record weight and height

6. Short Synacthen Test should be booked with the Chemical Pathologist or Chemical Pathology Registrar.

Pediatric tubes must be RED for testosterone collection (no gel).

Becton Dickinson gel tubes are not recommended for the MS testosterone assay.

Testesterone levels are stable for up to 72hrs on the gel. Do not add retrospective tests after 72 hours.

Becton Dickinson 5 mL plain tubes (no gel) are preferred for testosterone collection.

Becton Dickinson (BD) 5 mL plain tubes (no gel) are preferred for testosterone collection.

Paediatric tubes must be RED and not the paediatric BD gel tubes.

Adult BD gel tubes are acceptable up to 72 hours as testosterone levels are stable up to 72hours on gel.

6 mL of blood is the absolute minimum volume required.

Do not collect samples on the day before a long weekend as the sample must reach the external laboratory within 3 days.

6 mL of blood is the absolute minimum volume required.

Collect samples Monday to Friday only as the sample must reach the external laboratory within 3 days.

Minimum of 10 grams of faeces required.