Pathology Specimen Handbook

Recent changes for all departments


 Recent changes for ACYL CARNITINES

Recent changes for ACYL CARNITINES
DateFieldChanged FromChanged To
5th August 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
Optional Container TypeHeparin Plasma (Gel)

 Recent changes for ADENOSINE DEAMINASE ACTIVITY

Recent changes for ADENOSINE DEAMINASE ACTIVITY
DateFieldChanged FromChanged To
27th August 2019Request GroupADEN (For Blood) ZADEN (For Fluids)ADEN (For Blood)
Optional Specimen TypeFluid
Optional Container TypeHeparin Whole Blood (No Gel)
Collection & Request Instructions

Pleural fluid may be used for this assay.

Pleural fluid is stable for 7 days at 4 C, spin and send supernatant at 4 C.

 

 

 

 Recent changes for AMYLASE (24 HOUR URINE)

Recent changes for AMYLASE (24 HOUR URINE)
DateFieldChanged FromChanged To
31st July 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
26th July 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL

 Recent changes for AMYLASE (TIMED URINE)

Recent changes for AMYLASE (TIMED URINE)
DateFieldChanged FromChanged To
31st July 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL

 Recent changes for BCRABL

Recent changes for BCRABL
DateFieldChanged FromChanged To
16th May 2019Minimum/Paediatric Volume1 mL2.5 mL
Preferred Container TypeEDTA Whole Blood
EDTA Bone Marrow
PAXgene (Blood RNA)
EDTA Bone Marrow
Optional Container TypeEDTA Whole Blood
Collection & Request Instructions

Please ensure that blood for molecular testing for BCRABL is collected in a separate dedicated sterile tube. Molecular specimens must NOT be put through any analysers. If unsure please call the Genetics and Molecular Pathology laboratory.

Tube handling must be minimized to prevent contamination.

Collections between Friday 2:30pm and Sunday 12pm must be avoided. Specimens collected after 12pm on Sundays must be stored at room temperature. Specimens more than 24 hours old cannot be tested and a recollection will be requested.

For collections that occur externally to the Clayton site, please ensure that specimens reach the Clayton laboratory by 4pm if collected on Fridays.

Please ensure that blood for molecular testing for BCRABL is collected in a separate dedicated sterile tube. Molecular specimens must NOT be put through any analysers. If unsure please call the Genetics and Molecular Pathology laboratory.

Tube handling must be minimized to prevent contamination.

For EDTA tubes collections between Friday 2:30pm and Sunday 12pm must be avoided.

EDTA specimens collected after 12pm on Sundays must be stored at room temperature.

EDTA specimens more than 24 hours old cannot be tested and a recollection will be requested. Sample in Paxgene tube is viable for 3 days at room temparature.  

For EDTA collections that occur externally to the Clayton site, please ensure that specimens reach the Clayton laboratory by 3pm if collected on Fridays.

FrequencyOn requestWeekly

 Recent changes for BILIRUBIN (NEONATE)

Recent changes for BILIRUBIN (NEONATE)
DateFieldChanged FromChanged To
9th July 2019Collection & Request InstructionsProtect from light

 Recent changes for BLOOD CULTURES

Recent changes for BLOOD CULTURES
DateFieldChanged FromChanged To
13th June 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
Collection & Request Instructions

Adults add 5-10ml blood.  Fill the aerobic bottle first then the anaerobic. Paeds add 1-5ml blood. For mycobacteria use single specific bottle (contact Microbiology). Use sterile technique

Adults add 5-10ml blood.  Fill the aerobic bottle first then the anaerobic. Paeds add 1-5ml blood. For mycobacteria/Fungi use single specific bottle (contact Microbiology). Use sterile technique

 Recent changes for CATECHOLAMINES (24 HOUR URINE)

Recent changes for CATECHOLAMINES (24 HOUR URINE)
DateFieldChanged FromChanged To
1st July 2019Request GroupUMETAN UCRT (Include UCATM if a paediatric sample)UMETAN UCRT
Volume (Adults)null mL
Minimum/Paediatric Volumenull mL

 Recent changes for CATECHOLAMINES (SPOT URINE)

Recent changes for CATECHOLAMINES (SPOT URINE)
DateFieldChanged FromChanged To
11th July 2019Request GroupUMETAN UCRS (Include UCATMCR if a paediatric sample)UMETAN UCRS

 Recent changes for CATECHOLAMINES (TIMED URINE)

Recent changes for CATECHOLAMINES (TIMED URINE)
DateFieldChanged FromChanged To
11th July 2019Request GroupUMETAN UCRT (Include UCATM if a paediatric sample)UMETAN UCRT
Volume (Adults)null mL
Minimum/Paediatric Volumenull mL

 Recent changes for CEFAZOLIN

Recent changes for CEFAZOLIN
DateFieldChanged FromChanged To
19th July 2019Optional Container TypeHeparin Plasma (No Gel)
Serum (No Gel)
Heparin Plasma (No Gel)

 Recent changes for CEPHALEXIN

Recent changes for CEPHALEXIN
DateFieldChanged FromChanged To
19th July 2019Optional Container TypeHeparin Plasma (No Gel)

 Recent changes for Creutzfeld Jacob Disease

Recent changes for Creutzfeld Jacob Disease
DateFieldChanged FromChanged To
12th August 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL

 Recent changes for DNA - MYOTONIC DYSTROPHY

Recent changes for DNA - MYOTONIC DYSTROPHY
DateFieldChanged FromChanged To
29th August 2019Alternative namesMYOTONIC DYSTROPHYMYOTONIC DYSTROPHY, DM1 Gene
Request GroupMISCGENGMPTRAN

 Recent changes for FATTY ACIDS - FREE

Recent changes for FATTY ACIDS - FREE
DateFieldChanged FromChanged To
13th September 2019Preferred Container TypeFluoride OxalateFluoride Oxalate
Serum - Gel tube - Contains Clot Activator

 Recent changes for FOLATE (RED CELL)

Recent changes for FOLATE (RED CELL)
DateFieldChanged FromChanged To
25th September 2019Minimum/Paediatric Volumenull mL
Collection & Request Instructions

SERUM folate is the prefered test for folate status.

Red Cell Folate will only be done if specifically justified after discussion with the Clinical Haematologist or Chemical Pathologist.

SERUM folate is the prefered test for folate status.

 

 Recent changes for GASTRIN

Recent changes for GASTRIN
DateFieldChanged FromChanged To
15th July 2019Request GroupGASTGAST, MISCBIO
FrequencyWeekly

 Recent changes for KEPPRA

Recent changes for KEPPRA
DateFieldChanged FromChanged To
22nd August 2019Preferred Container TypeSerum (No Gel)Heparin Plasma (No Gel)
Optional Container TypeHeparin Plasma (No Gel)
Collection & Request Instructions

Trough level 8 hours post dose

MUST be non gel serum tube

Trough (pre-dose) level 8 hours post oral dose

MUST be non gel lithium heparin tube

 Recent changes for LEUKAEMIA DNA ANALYSIS

Recent changes for LEUKAEMIA DNA ANALYSIS
DateFieldChanged FromChanged To
16th October 2019Alternative namesFIP1L1-PDGFRA, PDGFRA, TCR GENE REARRANGEMENT, IGH GENE REARRANGEMENT, FLT3, AML1-ETO (t8:21), BCL2 (t14:18), BCL1 (t11:14), CBFB-MYHII (Inv 16), INV 16 QPCR, C-KIT, NPM1, T CELL GENE REARRANGEMENT, TP53 Mutation, AML SEQUENOMFIP1L1-PDGFRA, PDGFRA, TCR GENE REARRANGEMENT, IGH GENE REARRANGEMENT, FLT3, AML1-ETO (t8:21), BCL2 (t14:18), BCL1 (t11:14), CBFB-MYHII (Inv 16), INV 16 QPCR, C-KIT, NPM1, T CELL GENE REARRANGEMENT, TP53 Mutation, AML SEQUENOM, t(15;17) PML-PARA

 Recent changes for LINEZOLID

Recent changes for LINEZOLID
DateFieldChanged FromChanged To
30th August 2019Collection & Request Instructions

Collect 2 EDTA tubes.

Collect 2 EDTA tubes just before the next dose (trough collection) at least 48 hours after initiation or dose change (to reach steady state).

 Recent changes for Miscellaneous molecular

Recent changes for Miscellaneous molecular
DateFieldChanged FromChanged To
9th July 2019Optional Specimen TypeTissue - Fixed (Formalin)
Tissue - Fresh
Tissue - Frozen

 Recent changes for MYCOBACTERIUM TUBERCULOSIS PCR for Non-Respiratory Samples

Recent changes for MYCOBACTERIUM TUBERCULOSIS PCR for Non-Respiratory Samples
DateFieldChanged FromChanged To
5th September 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
5th September 2019Alternative namesTB PCR, MTB PCR
Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
5th September 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
Preferred Specimen TypeTissue - Fresh
Fluid
Bone Marrow
Cerebro Spinal Fluid (CSF)
Tissue - Fresh
Fluid
Bone Marrow
Cerebro Spinal Fluid (CSF)
Urine

 Recent changes for MYCOBACTERIUM TUBERCULOSIS PCR for Respiratory Samples

Recent changes for MYCOBACTERIUM TUBERCULOSIS PCR for Respiratory Samples
DateFieldChanged FromChanged To
5th September 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL

 Recent changes for MYCOPHENOLATE

Recent changes for MYCOPHENOLATE
DateFieldChanged FromChanged To
12th July 2019Collection & Request Instructions

Sample must be collected on ice.

Pre dose measurements are preferred.

However some requests may require 4 samples for area under the curve calculation.

Pre dose  

1hr post dose

2hrs post dose

4 hrs post dose

Requesting doctors must fill in a questionnaire of patient information

https://monashpathology.org/wp-content/uploads/2019/07/Mycophenolic_Acid.pdf

Sample must be collected on ice.

Requesting doctors must fill in a questionnaire of patient information

https://monashpathology.org/wp-content/uploads/2019/07/Mycophenolic_Acid.pdf

11th July 2019Collection & Request Instructions

Sample must be collected on ice.

Pre dose measurements are preferred.

However some requests may require 4 samples for area under the curve calculation.

Pre dose  

1hr post dose

2hrs post dose

4 hrs post dose

Sample must be collected on ice.

Pre dose measurements are preferred.

However some requests may require 4 samples for area under the curve calculation.

Pre dose  

1hr post dose

2hrs post dose

4 hrs post dose

Requesting doctors must fill in a questionnaire of patient information

 Recent changes for QUAD TEST

Recent changes for QUAD TEST
DateFieldChanged FromChanged To
4th June 2019Alternative namesMATERNAL SERUM TEST, TRIPLE TEST: FOR DOWNS SYNDROME & NTD, FIRST TRIMESTER SERUM SCREEN, PAPP-A (QUAD), Pre-eclampsia screenMATERNAL SERUM TEST, TRIPLE TEST: FOR DOWNS SYNDROME & NTD, FIRST TRIMESTER SERUM SCREEN, PAPP-A, PAPA (Pregnancy-Associated Plasma Protein A)(QUAD), Pre-eclampsia screen
Minimum/Paediatric Volumenull mL

 Recent changes for QUANTIFERON-TB GOLD PLUS ASSAY

Recent changes for QUANTIFERON-TB GOLD PLUS ASSAY
DateFieldChanged FromChanged To
4th July 2019Collection & Request Instructions

Requires QuantiFERON-TB Gold Plus Blood Collection Tubes set of four. Collect 1 mL into each tube. Refer to photograph for order of draw. Mix gently by turning tube end-over end X10 ensuring that the entire inner surface of the tube has been coated in blood.

Collections are to be performed at Pathology Collection rooms. Send to laboratory immediately after collection.

Please collect from Monday to Friday between 0900-1600 hours only.

DO NOT STORE IN FRIDGE BEFORE SENDING

Requires QuantiFERON-TB Gold Plus Blood Collection Tubes set of four. Collect 1 mL into each tube. Refer to photograph for order of draw. Mix gently by turning tube end-over end X10 ensuring that the entire inner surface of the tube has been coated in blood.

Collections are to be performed at Pathology Collection rooms. Send to laboratory immediately after collection.

Please collect from Monday to Friday between 0900-1600 hours only.

Please answer question if patient is immunosuppressed.

If non-MBS then non CMBS test

DO NOT STORE IN FRIDGE BEFORE SENDING

 Recent changes for RABIES (LYSSA VIRUS) SEROLOGY

Recent changes for RABIES (LYSSA VIRUS) SEROLOGY
DateFieldChanged FromChanged To
4th July 2019Collection & Request Instructions

Non-CMBS test

 Recent changes for RABIES PCR

Recent changes for RABIES PCR
DateFieldChanged FromChanged To
4th July 2019Collection & Request InstructionsRequires dedicated blood sample

Requires dedicated blood sample

Non-CMBS test

 Recent changes for RAPID INFLUENZA TEST

Recent changes for RAPID INFLUENZA TEST
DateFieldChanged FromChanged To
29th May 2019Assay nameRAPID INFUENZAE TESTINGRAPID INFUENZA TEST
Alternative namesRAPID RSV TESTING, INFLUENZAE RAPID TESTING, RSV RAPID TESTINGRAPID RSV TEST, INFLUENZA RAPID TEST, RSV RAPID TEST
Volume (Adults)null mL
Minimum/Paediatric Volumenull mL

 Recent changes for SEMEN ANALYSIS (INFERTILITY)

Recent changes for SEMEN ANALYSIS (INFERTILITY)
DateFieldChanged FromChanged To
1st June 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
Collection & Request Instructions

Patients (rather than samples) should be referred to the Andrology at Clayton Phone 9543 2833 or Richmond Phone 8540 5587 . Note patients are required to ring for an appointment

Patients (rather than samples) should be referred to the Andrology at Clayton Phone 8540 5587 or Richmond Phone 8540 5587 . Note patients are required to ring for an appointment

1st June 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
Collection & Request Instructions

Patients (rather than samples) should be referred to the Andrology at Clayton Phone 9590 8325 or Richmond Phone 9429 4603. Note patients are required to ring for an appointment

Patients (rather than samples) should be referred to the Andrology at Clayton Phone 9543 2833 or Richmond Phone 8540 5587 . Note patients are required to ring for an appointment

 Recent changes for SEMEN ANALYSIS (POST VASECTOMY)

Recent changes for SEMEN ANALYSIS (POST VASECTOMY)
DateFieldChanged FromChanged To
1st June 2019Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
Collection & Request Instructions

Patients (rather than samples) should be referred to the Andrology at Clayton Phone 9590 8325 or Richmond Phone 9429 4603. Note patients are required to ring for an appointment

Patients (rather than samples) should be referred to the Andrology at Clayton Phone 8540 5587 or Richmond Phone 8540 5587. Note patients are required to ring for an appointment

 Recent changes for STICKY TAPE TEST

Recent changes for STICKY TAPE TEST
DateFieldChanged FromChanged To
16th July 2019Alternative namesAnal Impression SmearsAnal Impression Smears; Pin Worm
Volume (Adults)null mL
Minimum/Paediatric Volumenull mL
Collection & Request Instructions

Collect specimen from the perianal area first thing in the morning, before the patient has bathed or used the toilet. The test should be performed for at least  4-6 consecutive days with negative results before a patient is considered free of infection.

A. Place a segment of sticky tape (adhesive side down) on a microscope slide: starting 1.5cm from one end, run the tape towards the same end, and wrap the tape around the slide to the opposite end. Tear the tape even with the end of the slide. Attach a label to the tape at the end torn flush with the slide.

B. Peel back the tape by gripping the labeled end and with the tape looped (adhesive side outward) over a wooden tongue depressor that is held firmly against the slide and extended about 2.5cm beyond it, press the tape firmly several times against the right and left perianal folds.

C. Smooth the tape back on the slide, adhesive side down. 

Collect specimen from the perianal area first thing in the morning, before the patient has bathed or used the toilet. The test should be performed for at least  4-6 consecutive days with negative results before a patient is considered free of infection.

A. Place a segment of sticky tape (adhesive side down) on a microscope slide: starting 1.5cm from one end, run the tape towards the same end, and wrap the tape around the slide to the opposite end. Tear the tape even with the end of the slide. Attach a label to the tape at the end torn flush with the slide.

B. Peel back the tape by gripping the labeled end and with the tape looped (adhesive side outward) over a wooden tongue depressor that is held firmly against the slide and extended about 2.5cm beyond it, press the tape firmly several times against the right and left perianal folds.

C. Smooth the tape back on the slide, adhesive side down. 

 Recent changes for TOXOPLASMA SEROLOGY

Recent changes for TOXOPLASMA SEROLOGY
DateFieldChanged FromChanged To
27th June 2019Request GroupTOXG,TOXMTOXGM (Acute), TOXG (IgG Screen only) TOXM (Infant ToRCH)
FrequencyDailyMon-Fri

 Recent changes for TREPONEMA PALLIDUM SEROLOGY

Recent changes for TREPONEMA PALLIDUM SEROLOGY
DateFieldChanged FromChanged To
27th June 2019FrequencyTwice weeklyMon-Fri
Uncontrolled when downloaded or printed.
Date printed: 19th October, 2019 10:38:35