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Recent changes for ACYL CARNITINES
Recent changes for ADENOSINE DEAMINASE ACTIVITY
Pleural fluid may be used for this assay.
Pleural fluid is stable for 7 days at 4 C, spin and send supernatant at 4 C.
Recent changes for AMYLASE (24 HOUR URINE)
Recent changes for AMYLASE (TIMED URINE)
Recent changes for BILIRUBIN (NEONATE)
Recent changes for BRAF V600E mutation test (melanoma)
Internal requests - BRAF molecular testing is requested in reflex manner on a 'Molecular Oncology Request Form' by the reporting Anatomical Pathologists. Non-pathology staff (i.e. oncologists, surgeons, etc) should contact the reporting Anatomical Pathologist if BRAF molecular test needs to be added to a case.
External requests - GMP laboratory will only process requests for BRAF testing for patients who had their histology performed at Monash Pathology. Please provide patient's Medicare number for billing purposes. MBS applies to Stage III and IV melanoma. Tests on other tumour types will be charged at the MBS schedule rate.
If your request is not covered by the above categories please contact the laboratory on 03 9594 3840.
Internal requests - BRAF molecular testing is requested in a reflex manner on a 'Molecular Oncology Request Form' by the reporting Anatomical Pathologists. Non-pathology staff (i.e. oncologists, surgeons, etc) should contact the reporting Anatomical Pathologist if BRAF molecular test needs to be added to a case.
Requesting clinicians must clearly indicate the test requested with appropriate clinical information. Clinical information must include the accession number of the histology episode on which the test is to be performed and any other relevant clinical information. Please fax requests to Monash Pathology on 9594 6619. Specimens will be retrieved from Histology and forwarded to Genetics and Molecular Pathology for testing. Monash Pathology will only process requests for patients who have had their histology performed at Monash Pathology. To avoid the patient receiving a bill for this test the patient’s Medicare number with the patient’s signature must be provided. The MBS currently only applies for tests on Stage III and IV melanoma. Tests on other tumours will be charged at the MBS schedule rate.
Recent changes for CATECHOLAMINES (24 HOUR URINE)
Recent changes for CATECHOLAMINES (SPOT URINE)
Recent changes for CATECHOLAMINES (TIMED URINE)
Recent changes for CEFAZOLIN
Recent changes for CEPHALEXIN
Recent changes for Creutzfeld Jacob Disease
Recent changes for DNA - CONNEXIN 26
Recent changes for DNA - MYOTONIC DYSTROPHY
Recent changes for FATTY ACIDS - ESSENTIAL
Patient should fast 12 hours prior to sample collection.
The patient must not consume any alcohol for 24 hours before the specimen is drawn.
Recent changes for FATTY ACIDS - FREE
Recent changes for FOLATE (RED CELL)
SERUM folate is the prefered test for folate status.
Red Cell Folate will only be done if specifically justified after discussion with the Clinical Haematologist or Chemical Pathologist.
Recent changes for GASTRIN
Recent changes for HERPES SIMPLEX VIRUS (HSV- type 1/2) PCR
Herpetic lesion: Wipe vesicle with saline. Disrupt vesicle, and collect fluid with dry PCR swab. With the same swab, collect cells from base of lesion. For non-vesicular lesions, collect cells from base of lesion by using swab moistened with saline. Specimens can be collected from vesicles from a variety of sites including genital. Refer to Herpes Multiplex PCR for CSF samples.
For other specimens collect as clinically indicated. This may include: tissue, CSF, saliva swab, urine, BAL and blood (PPT tube for adults, EDTA for paediatrics). Blood samples require a dedicated blood tube.
Collect Bronchial Washings, BAL, Lung Biopsy or Induced Sputum into sterile container. Specific requests and clinical notes are required. Expectorated sputum is not an appropriate specimen for detection of Pneumocystis.
Recent changes for KEPPRA
Trough level 8 hours post dose
MUST be non gel serum tube
Trough (pre-dose) level 8 hours post oral dose
MUST be non gel lithium heparin tube
Recent changes for LEUKAEMIA DNA ANALYSIS
Recent changes for LINEZOLID
Collect 2 EDTA tubes.
Collect 2 EDTA tubes just before the next dose (trough collection) at least 48 hours after initiation or dose change (to reach steady state).
Recent changes for Miscellaneous molecular
Recent changes for MYCOBACTERIUM TUBERCULOSIS PCR for Non-Respiratory Samples
Recent changes for MYCOBACTERIUM TUBERCULOSIS PCR for Respiratory Samples
Recent changes for MYCOPHENOLATE
Sample must be collected on ice.
Pre dose measurements are preferred.
However some requests may require 4 samples for area under the curve calculation.
1hr post dose
2hrs post dose
4 hrs post dose
Requesting doctors must fill in a questionnaire of patient information
Recent changes for PNEUMOCYSTIS JIROVECI PCR
Collect Bronchial Washings, BAL, Lung Biopsy or Induced Sputum into sterile container. Specific requests and clinical notes are required.
Recent changes for QUANTIFERON-TB GOLD PLUS ASSAY
Requires QuantiFERON-TB Gold Plus Blood Collection Tubes set of four. Collect 1 mL into each tube. Refer to photograph for order of draw. Mix gently by turning tube end-over end X10 ensuring that the entire inner surface of the tube has been coated in blood.
Collections are to be performed at Pathology Collection rooms. Send to laboratory immediately after collection.
Please collect from Monday to Friday between 0900-1600 hours only.
DO NOT STORE IN FRIDGE BEFORE SENDING
Please answer question if patient is immunosuppressed.
If non-MBS then non CMBS test
Recent changes for RABIES (LYSSA VIRUS) SEROLOGY
Recent changes for RABIES PCR
Requires dedicated blood sample
Recent changes for STICKY TAPE TEST
Collect specimen from the perianal area first thing in the morning, before the patient has bathed or used the toilet. The test should be performed for at least 4-6 consecutive days with negative results before a patient is considered free of infection.
A. Place a segment of sticky tape (adhesive side down) on a microscope slide: starting 1.5cm from one end, run the tape towards the same end, and wrap the tape around the slide to the opposite end. Tear the tape even with the end of the slide. Attach a label to the tape at the end torn flush with the slide.
B. Peel back the tape by gripping the labeled end and with the tape looped (adhesive side outward) over a wooden tongue depressor that is held firmly against the slide and extended about 2.5cm beyond it, press the tape firmly several times against the right and left perianal folds.
C. Smooth the tape back on the slide, adhesive side down.
Recent changes for TESTING
Recent changes for TOXOPLASMA SEROLOGY
Recent changes for TREPONEMA PALLIDUM SEROLOGY